Simply put…. yes.
Pennsylvania requires most fisherman to have a PA fishing license to fish the fresh water and brackish water and, well, just about any water 
in Pennsylvania.
Getting a PA fishing license is easy. You might not have to get one, I’d suggest checking with PA authorities or PA fish and game sites for current laws and updates on who needs a PA license to fish.
I wrote an article on the process of getting a license to fish in PA. You can do it write on line and print it out at home. Resident fishing licenses, non-resident fishing licenses, etc.
Check it out.
http://hubpages.com/hub/How-To-Obtain-PA-Fishing-License-Buy-Online-Easy
Please also read some of my other TurtleDog posts if you get a chance.