The production of high quality chocolate, and the farmers who grow it, will benefit from the recent sequencing and assembly of the chocolate tree genome, according to an international team led by Claire Lanaud of CIRAD, France, with Mark Guiltinan of Penn State, and including scientists from 18 other institutions.
The team sequenced the DNA of a variety of Theobroma cacao,moncler uk sale considered to produce the world’s finest chocolate. The Maya domesticated this variety of Theobroma cacao, Criollo, about 3,000 years ago in Central America, and it is one of the oldest domesticated tree crops. Today, many growers prefer to grow hybrid cacao trees that produce chocolate of lower quality but are more resistant to disease.
“Fine cocoa production is estimated to be less than 5 percent of the world cocoa production because of low productivity and disease susceptibility,” said Guiltinan,Moncler Girl Jackets professor of plant molecular biology.
The researchers report in the current issue of Nature Genetics “consumers have shown an increased interest for high-quality chocolate made with cocoa of good quality and for dark chocolate, containing a higher percentage of cocoa,moncler usa sale while also taking into account environmental and ethical criteria for cocoa production.”
Currently, most cacao farmers earn about $2 per day, but producers of fine cacao earn more.moncler vest sale Increasing the productivity and ease of growing cacao can help to develop a sustainable cacao economy. The trees are now also seen as an environmentally beneficial crop because they grow best under forest shade, allowing for land rehabilitation and enriched biodiversity.
The team’s work identified a variety of gene families that may have future impact on improving cacao trees and fruit either by enhancing their attributes or providing protection from fungal diseases and insects that effect cacao trees.
“Our analysis of the Criollo genome has uncovered the genetic basis of pathways leading to the most important quality traits of chocolate — oil,moncler womens hoody flavonoid and terpene biosynthesis,” said Siela Maximova, associate professor of horticulture, Penn State, and a member of the research team. “It has also led to the discovery of hundreds of genes potentially involved in pathogen resistance, all of which can be used to accelerate the development of elite varieties of cacao in the future.”
Because the Criollo trees are self-pollinating, they are generally highly homozygous, possessing two identical forms of each gene, making this particular variety a good choice for accurate genome assembly.
The researchers assembled 84 percent of the genome identifying 28,798 genes that code for proteins.moncler hatneckcloth They assigned 88 percent or 23,529 of these protein-coding genes to one of the 10 chromosomes in the Criollo cacao tree. They also looked at microRNAs, short noncoding RNAs that regulate genes, and found that microRNAs in Criollo are probably major regulators of gene expression.
“Interestingly, only 20 percent of the genome was made up of transposable elements, one of the natural pathways through which genetic sequences change,” said Guiltinan “They do this by moving around the chromosomes, changing the order of the genetic material.moncler bath towels Smaller amounts of transposons than found in other plant species could lead to slower evolution of the chocolate plant, which was shown to have a relatively simple evolutionary history in terms of genome structure.”