MOTECIINOLOGY

David (2005) while working with rose cultivars of different ploidy levels, analyzer’ the regeneration ability of a range of roses varieties as well as progeny indicated that this regenerative ability is genetically controlled. Unfortunately, most cultivars do riot regenerate readily.

Regeneration and transformation techniques have been developed for roses (Short and Roberts, 1991), Robinson and Firoozabady (1993), Chakrabarty etre. (2000) and David (2005). The somatic embryogenic callus line isolated by Noriega and Sondhal (1991) from the hybrid tea rose cultivars ‘Royalty has been transformed by Firrozabady Cl. al., (1594). They co-cultivated the callus with A. honefilciees L0A4454/pB3931 (pros NPTI)p3SLIJC or A. Atizogenes 15834/pJJ3499 (pons NRi’ll/p35SGUS). Selection of transformed callus was performed using 300 mg nit kanarnycin. Traiugenic plants regenerated hum the independent transformed calluses were shown by PCR to contain the t ran sgene. All plant produced were reported to be morphologically normal.

R. persica tr xantlibta has also been transformed via co-cultivation of protoplast with A. honefticiens LBA44191 using hygromycin as the selective agent. Transformed callus that stably expresses the GUS gene has been produced (Robimon and llireezabaily. 1993). Many workers have successfully transferred roses, however still with your efficiency. There is only one another report on successful establishment of long-term, repetitive ern bryogenic cultures of rose (Nonage and Sondahl, 1991).

The protocol of Noreiga and Sondahl (1991) was effectively utilized for genetic transformation of rose hy a novel Agrobacterium mediated system and about RIO of these regenerated, transgenic roses have flowered in the green house (Firoozabarly el. al., 1994). They obtained transgenic rose plantlets from embryogenic callus of ‘Royalty’ but again indicated that induction of erabryogenic tissues in rose culLivars was a rare event.

Vander Slam Cl. a/.(1997) reported production of ROIL gene transformed plants of Rosa Itybrida I.. and characterization of their rooting ability Won studied by them in detail. They regenerated transgenic plants from roots derived fret, stem slices of root stock R. hybrid co.’ Money Way’ following co-cultivation will, A. totenfiteiens strain. GV310/ containing the nptt/ gene and individual rot genes from A. et:it:news.

Merchant et. al, (1998) reported biolistic transform:Wirer of rose. A repioducible method has been developed for the brolistic transformation and regeneration of transgenic plants from embryugenic culture of rose (Rosa Itybritla L.) cy’Glad Tidings’. In this case DNA delivery was optimized using 13- glucuronidasc (Gus) gene. The distance behasai the stopping screen and target explants and supplimentation of pre and post borebarilimmt culture media will 0.25m rnyo-inositol influence the transformation efficiency. Prior to selection of culture media containing 250ing Kananlycin sulphate, embryogunic calli were bombarded, using optimized gene delivery parameters, wills a plasinid carrying Ore neomycin phosphotransferase (npl Ili gene. Somatic embryo derived kanainycin-resistant plants were regenerated and subsequently transferred to glass house conditions. Kanarnycin resistance of calli and plants confirmed transformation. NIT II HASA assay and southern analysis. All transgenic plants were morphologically normal anr1 hue to type.

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